2015م - 1444هـ
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Table of Contents

Chapter: Polymerase Chain Reaction

 Introduction

 Components of PCR

 Template

 Primers

 Properties of primers

 DNA polymerase

 dNTPs (deoxynucleotide triphosphates)

 Buffer

 Divalent cations

 Thermal cycler

 Procedure

 Denaturation

 Annealing

 Extension

 Variants of PCR

 Long accurate- PCR (LA-PCR)

 Inverse PCR

 Hot Start PCR

 Nested PCR

 Real time-q PCR

 RT- PCR (Reverse Transcriptase PCR)

 Multiplex PCR

 Applications

 Diagnosis of Genetic diseases

 Detection of the bacterial and viral infections

 Forensic studies

 Research laboratories

 Gene cloning

 Identification of transgenics

 Gene expression studies

 DNA Sequencing

 Marker-assisted plant breeding