2015م - 1444هـ
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Table of Contents
Chapter: Polymerase Chain Reaction
Introduction
Components of PCR
Template
Primers
Properties of primers
DNA polymerase
dNTPs (deoxynucleotide triphosphates)
Buffer
Divalent cations
Thermal cycler
Procedure
Denaturation
Annealing
Extension
Variants of PCR
Long accurate- PCR (LA-PCR)
Inverse PCR
Hot Start PCR
Nested PCR
Real time-q PCR
RT- PCR (Reverse Transcriptase PCR)
Multiplex PCR
Applications
Diagnosis of Genetic diseases
Detection of the bacterial and viral infections
Forensic studies
Research laboratories
Gene cloning
Identification of transgenics
Gene expression studies
DNA Sequencing
Marker-assisted plant breeding
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Plant Biotechnology Polymerase Chain Reaction PCR
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كتاب
Plant Biotechnology Polymerase Chain Reaction PCR
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