2010م - 1444هـ
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Epstein-Barr Virus

The B95-8 Strain Map

Paul J. Farrell

1. Introduction

This chapter summarizes the genes and mRNAs that have been mapped on to the

B95-8 EBV genome. The complete sequence of this strain of Epstein-Barr Virus (EBV)

was established (1,2) and data from many publications has been integrated into the

map, which is an update of that published previously (3). The B95-8 strain grows well

in laboratory culture and transforms human B lymphocytes efficiently, but B95-8 EBV

has about 11.8 kb deleted relative to other strains of EBV. The sequence of that region

has been determined in the Raji strain of EBV and a map has been published (4).

Detailed literature citations for most of the features have been published (3). The map

should be used in conjunction with the B95-8 EBV DNA sequence, which can be

accessed in the European Molecular Biology Laboratory (EMBL; embl:ebv.seq) or

Genbank databases. The feature tables shown in those database files have not yet been

revised so the information shown in this map is considerably more up to date.

2. Repeat Sequences

There are several regions of the genome that contain tandem repeat sequences.

Some of these are large repeat units (for example, the major internal repeat is 3072 bp

and the terminal repeat is 538 bp) but some are much simpler repeats. In most virus

preparations, there will be a distribution of copy number of these repeats, so it is important to appreciate that the viral map and coordinates are just one reference example of

genome structure. Generally the repeat numbers in the B95-8 map are thought to be

typical, although it now appears that the 11.5 copies of the major internal repeat

inserted into the B95-8 sequence may be an over-estimate, about 8.5 being more usual

in B95-8 EBV (5)